We are pleased to present the third issue of JIOHS, which focuses on optical technologies of diagnostics and therapy. These are selected papers presented during Saratov Fall Meeting — 1st International Symposium on Optics and Biophotonics (September 24–28, 2013, Saratov, Russia) in the framework of the Workshops: “Optical Technologies in Biophysics and Medicine," “Nanobiophotonics," and “Internet Biophotonics." This issue includes nine representative papers that well characterize the major topics of SFM-13.