Raman spectroscopy has been applied for rapid analysis of ganoderma spore oil. It is found that the Raman spectrum of ganoderma spore oil has a weak and broad characteristic phonon mode at 1 563 cm-1, which is not observed in olive oil, sunflower seed oil, and cod-liver oil. In addition, the relative intensity ratio of the two phonon modes at 1 445 and 1 660 cm-1 in ganoderma spore oil is very different comparing with that in olive oil, sunflower seed oil, and cod-liver oil. Furthermore, Raman analysis of deteriorated ganoderma spore oil and fake ganoderma spore oil is investigated. Our results showed that when ganoderma spore oil is exposed in air, its active ingredients will be oxidized, and the fake ganoderma spore oil on market could be a mixture of deteriorated ganoderma spore oil, sunflower seed oil, or other cheap vegetable oils.