• Spectroscopy and Spectral Analysis
  • Vol. 41, Issue 12, 3727 (2021)
Tao CHEN1、1;, Hui GUO1、1;, Man YUAN1、1;, Fu-yuan TAN3、3; *;, Yi-zhou LI2、2; *;, and Meng-long LI1、1;
Author Affiliations
  • 11. College of Chemistry, Sichuan University, Chengdu 610064, China
  • 22. School of Cyber Science and Engineering, Sichuan University, Chengdu 610064, China
  • 33. Biological Process Science and Technology Co., Ltd., Chengdu 610093, China
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    DOI: 10.3964/j.issn.1000-0593(2021)12-3727-06 Cite this Article
    Tao CHEN, Hui GUO, Man YUAN, Fu-yuan TAN, Yi-zhou LI, Meng-long LI. Recognition of Different Parts of Wild Cordyceps Sinensis Based on Infrared Spectrum[J]. Spectroscopy and Spectral Analysis, 2021, 41(12): 3727 Copy Citation Text show less
    The averaged Fourier-transform infrared spectra for different parts of Cordyceps (a); The similarities between each two parts of Cordyceps (b)
    Fig. 1. The averaged Fourier-transform infrared spectra for different parts of Cordyceps (a); The similarities between each two parts of Cordyceps (b)
    The confusion matrix of independent data set by different methods(a): CARS-PLS-DA; (b): CARS-LDA; (c): VCPA-PLS-DA; (d): VCPA-LDA
    Fig. 2. The confusion matrix of independent data set by different methods
    (a): CARS-PLS-DA; (b): CARS-LDA; (c): VCPA-PLS-DA; (d): VCPA-LDA
    The result of feature selection method CARS (a) and VCPA (b)
    Fig. 3. The result of feature selection method CARS (a) and VCPA (b)
    The box-plot A (1 084, 1 024, 630, 879 cm-1) and heat-map for Wilcoxon rank-sum test of wavenumbers selectedby CARS B(1 084, 1 024, 630, 879 cm-1)
    Fig. 4. The box-plot A (1 084, 1 024, 630, 879 cm-1) and heat-map for Wilcoxon rank-sum test of wavenumbers selectedby CARS B(1 084, 1 024, 630, 879 cm-1)
    The box-plot A(1 089, 1 028, 874, 625 cm-1) and heat-map for Wilcoxon rank-sum test of wavenumbers selected by VCPA B(1 089, 1 028, 874, 625 cm-1)
    Fig. 5. The box-plot A(1 089, 1 028, 874, 625 cm-1) and heat-map for Wilcoxon rank-sum test of wavenumbers selected by VCPA B(1 089, 1 028, 874, 625 cm-1)
    OrgansHSMSHDMLEL
    Number of sample161162162162161
    Table 1. General information of samples
    Pre-treatment methodsMethods10fold-CV/%Independent test set/%Variable number
    SNV+MSCPLS-DA90.192.03 601
    SNV+MSCLDA86.785.83 601
    SNV+MSCCARS-PLS-DA90.090.1669
    SNV+MSCCARS-LDA76.380.9669
    SNV+MSCVCPA-PLS-DA89.691.4420
    SNV+MSCVCPA-LDA83.082.1420
    Table 2. The model performance on discriminating different cordyceps parts
    Base group and
    vibration mode
    CARS
    /cm-1
    VCPA
    /cm-1
    Base group and
    vibration mode
    CARS
    /cm-1
    VCPA
    /cm-1
    ν(O—H, N—H)
    in OH, NH
    3 389νas(C—H) in CH3, CH22 9212 927
    3 388νs(C—H) in CH3, CH22 8532 856
    3 3473 3872 855
    3 3453 372ν(C=O)in ester1 741
    3 3433 360ν(C=O) in Carboxy acid1 7061 703
    3 3383 358ν(CO) in Amide Ⅰ1 6581 658
    3 3293 3451 657
    3 3283 313δ(N—H) in Amide Ⅱ1 5411 545
    3 3253 306δ(C—H) in CH31 4591 449
    3 3213 304δs(C—H) in CH31 3811 373
    3 3163 302ν(C—OH) in Mannitol1 0841 089
    3 3113 2961 0241 028
    3 2963 285δ(C—O—C) in Saccharides, ring879874
    3 2843 280breathing
    3 282ν(C—C) in —(CH2)4716702
    3 280δ(C—O—H) in Mannitol, wagging630625
    Table 3. Holistic assignment of infrared spectroscopy spectra of Cordyceps
    Tao CHEN, Hui GUO, Man YUAN, Fu-yuan TAN, Yi-zhou LI, Meng-long LI. Recognition of Different Parts of Wild Cordyceps Sinensis Based on Infrared Spectrum[J]. Spectroscopy and Spectral Analysis, 2021, 41(12): 3727
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