For multipoint temperature measurement in various environments, a portable multichannel temperature data acquisition system was designed. Making use of the thermocouple as the temperature sensor, the signal of thermocouple is modulated by NI 9211 4channel thermocouple input module, then sent into the computer through the USB bus for analysis and display. Based on a graphic development environment of LabVIEW, and with the flexible configurations of all parameters, the system software has a good applicable value in the multipoint temperature measurement.