We realized hologram storage within 0.07 cm 2 light spot in thin layers of polyvinyl alcohol (PVA) matrices doped with methyl orange dyes (MO), pre irradiated by the 488.0 nm line of an Ar ion laser using two orthogonal linear polarized 632.8 nm light beams. By rotating sample, multi hologram storage was achieved. By controlling writing time, three hologram images have been recorded in the same light spot. The mechanism of the multiple hologram storage in MO/PVA thin film was discussed using a four energy level model of MO molecular in PVA materices.