The uncertainty of laboratory calibrated spectroradiometers used in field was analyzed. Ambient temperature is one of the most important factors limiting the accuracy of outdoor measurements. Currently, the laboratory calibrations are generally performed at room temperature ［(25±1.0) ℃］. However, the spectrometers applied in earth observation and remote sensing is usually operated in the field under different ambient temperature conditions. The calibration coefficients determined under room temperature are not applicable to data collected in field conditions. In this paper, the experimental measurement system was set up, which was used to investigate the temperature effects. The sensitivity of spectroradiometers (CR-280) at different wavelengths was affected differently by ambient temperature. The deviation of 400～700 nm between measured and calibrated value at 40° is about ±5%. The deviation of near-infrared wavelengths (1 050 nm) is about ±15%, this spectral range is close to the band edge of the silicon, which is highly temperature sensitive, and the silicon band edge moved to longer wavelengths as increasing temperature. It is important to reduce the deviation of measured results in field after laboratory calibration. Here, a temperature correction method by matrix calculation for different kinds of spectroradiometers was proposed, which calculated the spectroradiometer response at each pixel. The correction method was also verified with a randomly selected temperature. It can reduce the deviation of CR-280 from ±10% to ±1% in the near-infrared wavelengths (about 950 nm). The temperature correction method can be easily used for spectral radiometry measurement in the field, which can be greatly improves the accuracy of spectral radiometry measurement. Also, the other two kinds of spectroradiometers (Avantes, SVC H-1024) were used to verify this correction method. The result showed the deviation of Avantes (VIS/NIR) between measured and calibrated value (about 1 060 nm) is reduced from ±17% to ±1%. At different ambient temperatures, the deviations between the measured and calibrated values of SVC HR-1024 are different. The instrument consists of three detectors: Si, cooled InGaAs and extended InGaAs. Si detector is greatly affected by temperature, and the deviation of the measured and calibrated value (950～1 000 nm) is as high as ±10%. The cooled InGaAs can effectively control the detector temperature. However, as the ambient temperature increases, the InGaAs detector is affected (the optimal working environment for cooling is about 20 ℃), and the deviation of measuredand calibrated value results is 1%~3%. The temperature correction formulas were used to correct the measured results. The deviation of SVC HR-1024 can be reduced to ±1%.