Three dimensional optical storage based on femtosecond laser is one of the most important methods to realize high density and super-high density optical storage. The absorption spectrum of a new micro explosion material(Sm0.5Ce0.5(DBM)3 Phen dye doped with PMMA as substrate) was measured and analyzed, the fluorescence spectra before and after excitation were obtained by using 514.5 nm laser as laser source, the characteristics of electron rotation resonance spectra before and after excitation were obtained by using 800 nm femtosecond laser as excitation source, the change of storage point size and storage point readout gray value under different femtosecond laser pulse energy was analyzed, the four layer optical information storage of femtosecond laser was realized, the distance between the points is 4 μm and the distance between layers is 16 μm. The experimental results show that this material can be well applied to 3D optical information storage.