The growth and spectroscopic properties of Tm3+Sr3La2(BO3)4 crystal are reported. Based on Judd-Ofelt theory, the intensity parameters are calculated to be Ω2=4.46×10-20 cm2, Ω4=3.18×10-20 cm2, and Ω6=1.88×10-20 cm2 according to the absorption spectrum. The emission cross section for the 3F4→3H6 transition are estimated to be 0.694×10-20 cm2 at 1.82 μm by the reciprocity method according to the absorption cross section, and the relation between gain cross section and the population inversion rate for the 3F4→3H6 transition is discussed. Compared with the other Tm3+-doped materials, Sr3La2(BO3)4 crystal has a larger emission cross section and wider tuning range. Therefore, Sr3La2(BO3)4 crystal may be a potential tunable near-infrared laser crystal.