In this study, TC4 titanium alloy bulk specimens are prepared using the laser three-dimensional(3D)printing technology. The effect of normalizing temperature on the laser 3D-printed TC4 titanium alloy microstructure, phase composition, and tensile properties at room temperature is studied via an optical microscope, scanning electron microscopy (SEM), X-ray diffractometer (XRD), and electronic universal testing machine. Experimental results reveal that the α-Ti phase recrystallizes and the length and width of the α-Ti phase increase after being normalized at the α-Ti phase zone. The microstructures mainly comprise α-Ti phase and a small amount (or trace amount) of the β-Ti phase. The tensile property at room temperature is general. After being normalized at the α+β phase zone, the slender primary α-Ti phase in the as-deposited state thrusts each other because of the β-Ti phase precipitation, transforming into a short rod-shaped primary α-Ti phase. The β-Ti phase not only coexists with the secondary α-Ti phase between the short rod-shaped primary α-Ti phases, but also precipitates and is distributed as networks inside the short rod-shaped primary α-Ti phase. After 990 ℃/2 h/AC treatment, the room-temperature tensile strength, yield strength, and elongation are 960 MPa, 835 MPa, and 17%, which satisfy the national standard for forging. The fracture morphology of the tensile specimens is covered with dimples, all of which are ductile fractures.