Dataset simulated with FluorMOD and images of wheat in heading stage taken by a ground-based hyperspectral imaging system with 3.3 nm spectral resolution and 0.71~0.74 nm spectral sampling interval were used test the feasibility and accuracy of three FLD methods (named FLD, 3FLD and iFLD). The results show that when spectral resolution is 3.3 nm, solar-induced chlorophyll fluorescence could be extracted effectively in O2-A band (around 760 nm) instead of O2-B band (around 687 nm). As to the extraction results of data with noises, both FLD and 3FLD are stabler than iFLD method. The results of FLD tend to be higher than true value.