To carry out the measurement of vehicle body position and dimension of loading robot before loading, an intelligent vehicle body measurement system based on two-dimensional LiDAR was provided, and the calibration method of this system was studied as a key point. The two-dimension LiDAR was driven by rotating the platform, and the three-dimensional information of car body measured was obtained by using the single two-dimensional laser radar. In allusion to the complexity of calibration method of LiDAR measurement system and the difficulty in making calibration pieces, a system parameter calibration method was proposed based on 321 coordinate system building method, and mathematical models of calibration was established, with the principle and procedure of calibration method in detail. Measurement system was set up in a laboratory to carry out calibration experiment and mea-surement experiment on simulation vehicle body, and the measurement experiment for real vehicle body was con-ducted outside. The experiment result shows that the maximum measurement error of vehicle body size and length of this measurement system was 26.4 mm; maximum angle measurement error was 0.18 degree, which fully meets the precision requirements of loading.