A new calibration method for laser plane with double virtual circles is put forward. This method creates double virtual circles in the image coordinate using the corners of chessboard. Position relationship is established between the virtual circles and the laser stripes. The feature point coordinates of the laser stripe in the camera coordinate system are calculated according to the principle of cross-ratio invariance. The chessboard position is changed for several times within the field of the camera vision aiming to calculate more feature points. All these feature points are fitted to an ideal plane by the least square method (LSM). The experimental results indicate that the precision is obviously higher than the actual circle target. The root mean square (RMS) error of the laser plane calibration is 0.04 mm and the target is easier to make. It concludes that the calibration process is simple, reliable and suits for calibration on spot.