• Laser & Optoelectronics Progress
  • Vol. 55, Issue 4, 043003 (2018)
Bo Yu1, Xiufeng Lan1、*, Lin Zhang1, Ruping Zou1, and Qi Chen1
Author Affiliations
  • 1 School of Science, Hohai University, Nanjing, Jiangsu 211100, China
  • 1 School of Science, Nanjing University of Aeronautics & Astronautics, Nanjing, Jiangsu 210016, China;
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    DOI: 10.3788/LOP55.043003 Cite this Article Set citation alerts
    Bo Yu, Xiufeng Lan, Lin Zhang, Ruping Zou, Qi Chen. Spectroscopic Study on Interaction of Famotidine with Bovine Serum Albumin[J]. Laser & Optoelectronics Progress, 2018, 55(4): 043003 Copy Citation Text show less
    Chemical structure of famotidine
    Fig. 1. Chemical structure of famotidine
    Fluorescence quenching spectra of famotidine-BSA system
    Fig. 2. Fluorescence quenching spectra of famotidine-BSA system
    Stern-Volmer plots of BSA interacted by famotidine
    Fig. 3. Stern-Volmer plots of BSA interacted by famotidine
    Fitted lgF0-F/F-lgQ curves at different temperatures
    Fig. 4. Fitted lgF0-F/F-lgQ curves at different temperatures
    Fluorescence emission spectrum of BSA and ultraviolet absorption spectrum of famotidine
    Fig. 5. Fluorescence emission spectrum of BSA and ultraviolet absorption spectrum of famotidine
    T/KLinear equationof Stern-VolmerCorrelationcoefficientQuenching constantKSV /(104 L·mol-1)Quenching rate constantKq /(L·mol-1·s-1)
    306F0/F=1.0128+7.773×104Q0.990237.773×1047.773×1012
    314F0/F=1.01207+6.15×104Q0.997546.15×1046.15×1012
    Table 1. Stern-Volmer equation, quenching constant and quenching rate constant of famotidine-BSA system
    T/KLinear equationCorrelationcoefficient R2Number ofbinding sites nApparent binding constantKA /(L·mol-1)
    306lgF0-F/F=4.99392+1.01904 lgQ0.995441.019049.861×104
    314lgF0-F/F=4.59005+0.95886 lgQ0.999260.958863.891×104
    Table 2. Apparent binding constant and number of binding sites of famotidine-BSA system
    T/KKA /(L·mol-1)ΔH /(kJ·mol-1)ΔG /(kJ·mol-1)ΔS /(J·mol-1·K-1)
    3069.861×104-93.149-29.254-208.78
    3143.891×104-93.149-27.591-208.78
    Table 3. Thermodynamic parameter table
    Bo Yu, Xiufeng Lan, Lin Zhang, Ruping Zou, Qi Chen. Spectroscopic Study on Interaction of Famotidine with Bovine Serum Albumin[J]. Laser & Optoelectronics Progress, 2018, 55(4): 043003
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