Nature reserves are the core area for biodiversity conservation. National nature reserves are the main body of nature reserves in China. According to characteristic of each type of human activity, the patches of different human activities of 446 national nature reserves in 2015 and their change from 2013 to 2015 were interpreted with the aid of GIS and RS. Status of the distribution of human activities and their change are generally analyzed in different functional zones and different regions in China. Also according to different types of human activities, the intensity and characteristics of dynamic change of human activities are evaluated from 2013 to 2015. The results showed that in 2015, there were different kinds of human activities in 446 national nature reserves. Human activities were widely distributed, and agricultural land and residential areas were dominant. The area of the two types covered more than 88.94% of the total of human activities. And the number exceeded 77.67% of the total. In China, 66.59% of national nature reserves had new or expanded human activities from 2013 to 2015, and the amount of human activities increased by 2.45%. Compared with agricultural land and residential areas, the increase trend is more pronounced of the development or construction activities. From 2013 to 2015, the number of national nature reserves where human activities had no change covered 33.41% of the total number of national nature reserves in China, those with little change covered 45.06%, and those with and significant change covered only 21.53%. From the perspective of spatial distribution, the overall human activity in the national nature reserves of eastern China is greater than that of central and western parts of the country. The intensity of human activities in coastal and riverside national nature reserves is relatively strong.