Xiao-qing XU, Qian ZHOU, Jian-hua SUN, Li-xia SUN, Xue-zhen FENG, Yong-fang XU, Zhang-fa TONG, Dan-kui LIAO. Study on the Inhibition Mechanism of Angiotensin Conversion Enzyme Inhibitor Peptide Leu-Lys-Pro[J]. Spectroscopy and Spectral Analysis, 2022, 42(7): 2107
- Spectroscopy and Spectral Analysis
- Vol. 42, Issue 7, 2107 (2022)
Fig. 1. Fluorescence emission spectra of ACE with LKP at different temperatures
(a)—(c): 288, 293, 298 K,c (ACE)= 1.0×10-6 mol·L-1
(a)—(c): 288, 293, 298 K,
Fig. 2. The Stern-Volmer plots of ACE with LKP
Fig. 3. UV absorption spectra of ACE with LKP at different temperatures
(a)—(c): 288, 293, 298 K,c (ACE)=1.0×10-6 mol·L-1,c (LKP)(0—4): 0, 0.28, 1.4, 2.8, 4.2×10-3 mol·L-1
(a)—(c): 288, 293, 298 K,
Fig. 4. CD spectra of ACE with LKP
298 K,c (ACE)= 1.0×10-6 mol·L-1
298 K,
Fig. 5. The heat flow of binding LKP to ACE
(a)—(c): 288, 293, 298 K,c (ACE)=0.4 μmol·L-1, c (LKP)=2.8 mmol·L-1
(a)—(c): 288, 293, 298 K,
Fig. 6. The docking simulation of LKP binding to ACE
(a): The docking simulation of LKP binding to ACE;(b): The interaction between LKP and the residues of ACE is shown
(a): The docking simulation of LKP binding to ACE;(b): The interaction between LKP and the residues of ACE is shown
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Table 1. Quenching constants of ACE with LKP at different temperatures
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Table 2. Thermodynamics parameter of binding of LKP and ACE
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