• Acta Photonica Sinica
  • Vol. 51, Issue 8, 0851517 (2022)
Yuehan ZHAO and Xiang HAO
Author Affiliations
  • [in Chinese]
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    DOI: 10.3788/gzxb20225108.0851517 Cite this Article
    Yuehan ZHAO, Xiang HAO. Advances in Multicolor Single-molecule Localization Microscopy(Invited)[J]. Acta Photonica Sinica, 2022, 51(8): 0851517 Copy Citation Text show less
    Schematic diagram of the imaging principle of Exchange-PAINT[29]
    Fig. 1. Schematic diagram of the imaging principle of Exchange-PAINT29
    Performance of globLoc on ratiometric multi-color data. 4 color 3D imaging of Nup62-DY634,Nup96-AF647,ELYS-CF660C and WGA-CF680 in the NPC[40]
    Fig. 2. Performance of globLoc on ratiometric multi-color data. 4 color 3D imaging of Nup62-DY634,Nup96-AF647,ELYS-CF660C and WGA-CF680 in the NPC40
    Dual-color imaging of microtubules and mitochondria using grating[42]
    Fig. 3. Dual-color imaging of microtubules and mitochondria using grating42
    The optical set-up of SR-STROMs
    Fig. 4. The optical set-up of SR-STROMs
    The optical system and Representation of the phase masks placed at the pupil plane[64]
    Fig. 5. The optical system and Representation of the phase masks placed at the pupil plane64
    Comparison of different Multicolor SMLM
    Fig. 6. Comparison of different Multicolor SMLM
    FluorophoreλemTargetTypeReferences
    Atto 520,AF647538 nm,665 nmNPCMore excitation20
    mEos2,AF647519 nm,665 nmPlasma membrane21
    Dronpa,AF647518 nm,665 nmHIV-1 virions2223
    AF405-Cy5,Cy2-Cy5,Cy3-Cy5

    421 nm,504 nm

    556 nm

    Microtubules and clathrin-coated pits,m-RNAActivator-reporter

    25

    27

    AF405-AF647665 nmMicrotubules and mitochondrial outer membrane proteinQuenching28
    AF488,AF514,Cy3

    519 nm,543 nm

    568 nm

    F-actin,clathrin,and tubulinRatiometric SMLM33
    AF647,DY634,CF660C,CF680

    665 nm,658 nm

    685 nm,698 nm

    Nup62,Nup96,ELYS and WGA in the NPC40
    Dy634,DL650,CF660C,CF680

    658 nm,673 nm

    685 nm,698 nm

    Peroxisomes,vimentin filaments,microtubules and the outer mitochondrial membraneSR-STORM41
    Table 1. Dye combinations used in different multicolor SMLM
    Yuehan ZHAO, Xiang HAO. Advances in Multicolor Single-molecule Localization Microscopy(Invited)[J]. Acta Photonica Sinica, 2022, 51(8): 0851517
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