Finally, we present an outlook on the fast-growing field of multicolor single-molecule localization microscopy. One of the future development directions of multicolor single molecules is the research on probes, including the brighter organic fluorescent dyes or fluorescent proteins and more universal imaging buffers. Further research can also be done on reducing the complexity of the optical system and expanding the field of view of imaging. It can be envisioned that multicolor SMLM will play a more prominent role in biological and medical research for many years to come. We hope that this review can help researchers choose the appropriate multicolor SMLM for their own experiments.