Effect of Photobiomodulation Mediated by 808 nm Laser on Active Oxygen Steady-State in CCC-ESFs under High Glucose Environment

Guang Han; Jingjing Gao; Mengru Tu; Jia Shi; Zhenhua Pan; Hongli Chen

doi:10.3788/LOP202158.0917001

Journals >Laser & Optoelectronics Progress >Volume 58 >Issue 9 >Page 0917001 > Article

Laser & Optoelectronics Progress
Vol. 58, Issue 9, 0917001 (2021)

Effect of Photobiomodulation Mediated by 808 nm Laser on Active Oxygen Steady-State in CCC-ESFs under High Glucose Environment

Guang Han^1、3, Jingjing Gao¹, Mengru Tu¹, Jia Shi^2、3, Zhenhua Pan⁴, and Hongli Chen^1、3、*

Author Affiliations

¹School of Life Sciences, Tiangong University, Tianjin 300387, China

²School of Electronic and Information Engineering, Tiangong University, Tianjin 300387, China

³Tianjin Key Laboratory of Photoelectric Detection Technology and Systems, Tianjin 300387, China

⁴Institute of Lung Cancer, General Hospital of Tianjin Medical University, Tianjin 300052, China

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DOI: 10.3788/LOP202158.0917001 Cite this Article Set citation alerts

Guang Han, Jingjing Gao, Mengru Tu, Jia Shi, Zhenhua Pan, Hongli Chen. Effect of Photobiomodulation Mediated by 808 nm Laser on Active Oxygen Steady-State in CCC-ESFs under High Glucose Environment[J]. Laser & Optoelectronics Progress, 2021, 58(9): 0917001 Copy Citation Text

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Fig. 1. Cell morphology observation results. （a） Normal medium; （b） high glucose medium

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Fig. 2. Absorbance of CCC-ESFs irradiated by 808 nm laser with different power densities (n=3;* means p<0.05, compared with high glucose control group)

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Fig. 3. Absorbance of CCC-ESFs irradiated by 808 nm laser with different energy densities (n=3; * means p<0.05, compared with high glucose control group;** means p<0.01, compared with high glucose control group)

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Fig. 4. Intracellular ROS level (n=3; * means p<0.05, compared with normal glucose control group; ** means p<0.01, compared with normal glucose control group; # means p<0.05, compared with high glucose control group; ## means p<0.01, compared with high glucose control group)

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Fig. 5. Total intracellular SOD level (n=3; * means p<0.05, compared with normal glucose control group; ** means p<0.01, compared with normal glucose control group; # means p<0.05, compared with high glucose control group)

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Fig. 6. TAC level of cells (n=3; * means p<0.05, compared with normal glucose control group; ** means p<0.01, compared with normal glucose control group; # means p<0.05, compared with high glucose control group; ## means p<0.01, compared with high glucose control group)

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Fig. 7. Intracellular mitochondrial membrane potential level

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Parameter	Value
Wavelength /nm	808
Output mode	Continuous
Average radiant power /mW	40
Spot area /cm²	2
Vertical distance /cm	4
Beam profile	Round
Beam divergence angle /(°)	90

Table 1. Irradiation parameters

Power density /(mW·cm^-2)	Energy density /(J·cm^-2)	Irradiation time /min
10	1.5	2.5
	3	5
	6	10
	12	20
20	1.5	1.25
	3	2.5
	6	5
	12	10
40	1.5	0.625
	3	1.25
	6	2.5
	12	5

Table 2. Laser irradiation dose

Item	NF-κB level /（pg·mL^-1）		TNF-α level /（pg·mL^-1）		IL-1β level /（pg·mL^-1）
Item	A₄₅₀	Standard deviation	A₄₅₀	Standard deviation	A₄₅₀	Standard deviation
Control-HG	1066.22	±6.81	74.11	±0.55	71.33	±0.55
Control-NG	1171.22	±10.86*	78.23	±0.37*	74.92	±0.15**
3 J·cm^-2-HG	1061.22	±66.35	75.72	±2.26**	66.48	±0.79**
3 J·cm^-2-NG	1111.22	±36.8^##	74.45	±0.96^#	69.72	±1.16^##

Table 3. NF-κB, TNF-α and IL-1β levels of cells irradiated by 808 nm laser

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