• Laser & Optoelectronics Progress
  • Vol. 58, Issue 24, 2410001 (2021)
Qing Chen, Haiyang Zhou, and Feihong Yu*
Author Affiliations
  • College of Optical Science and Engineering, Zhejiang University, Hangzhou, Zhejiang 310027, China
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    DOI: 10.3788/LOP202158.2410001 Cite this Article Set citation alerts
    Qing Chen, Haiyang Zhou, Feihong Yu. Microscopic Image Mosaic Algorithm Using Improved Optical Flow Method[J]. Laser & Optoelectronics Progress, 2021, 58(24): 2410001 Copy Citation Text show less
    Flow chart of proposed method
    Fig. 1. Flow chart of proposed method
    Image overlap area
    Fig. 2. Image overlap area
    Original images
    Fig. 3. Original images
    Stitching results. (a) Autostitch method; (b) template matching method; (c) optical flow method; (d) proposed method
    Fig. 4. Stitching results. (a) Autostitch method; (b) template matching method; (c) optical flow method; (d) proposed method
    Original images
    Fig. 5. Original images
    Point matching results before and after improvement
    Fig. 6. Point matching results before and after improvement
    Stitching results before and after improvement. (a)(c) Before improvement; (b)(d) after improvement
    Fig. 7. Stitching results before and after improvement. (a)(c) Before improvement; (b)(d) after improvement
    Correct matching rate before and after adding constraints
    Fig. 8. Correct matching rate before and after adding constraints
    Original images for mosaic test
    Fig. 9. Original images for mosaic test
    Results of mosaic test
    Fig. 10. Results of mosaic test
    Panorama of microscopic slices. (a) Overall stitching performance of proposed method; (b)-(d) stitching detail
    Fig. 11. Panorama of microscopic slices. (a) Overall stitching performance of proposed method; (b)-(d) stitching detail
    AlgorithmAutostitchTemplate matchingOptical flowProposed method
    Time /s3.7800.2780.2160.121
    Table 1. Running time of different algorithms
    GroupNumber of matchesNumber of matches after eliminate outlierNumber of correct matchesAccuracy /%
    113213012293.85
    13212311696.75
    21311205344.17
    131151173.33
    31211087266.67
    121544583.33
    412312010486.67
    123908695.56
    5108986162.24
    108332781.31
    6105978981.82
    105878496.55
    Table 2. Analysis of accuracy rate before and after adding constraints
    Qing Chen, Haiyang Zhou, Feihong Yu. Microscopic Image Mosaic Algorithm Using Improved Optical Flow Method[J]. Laser & Optoelectronics Progress, 2021, 58(24): 2410001
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