Runna Liu, Jing Liu, Hua Tian, Santing Zheng, Tingting Zheng. Design of a Miniaturized Cell Microscopic Image Acquisition System Based on Wireless Transmission[J]. Laser & Optoelectronics Progress, 2025, 62(2): 0211005

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- Laser & Optoelectronics Progress
- Vol. 62, Issue 2, 0211005 (2025)

Fig. 1. Design block diagram of miniaturized microscopic image acquisition system based on wireless transmission

Fig. 2. Pictures of miniaturized microscope. (a) Miniaturized microscope system; (b) COMS imaging camera; (c) physical drawing of support and its 3D model; (d) combination diagram of motherboard and objective lens; (e) physical drawing of base and its 3D model; (f) LED array; (g) WiFi module

Fig. 3. Intelligent microscopic image acquisition software. (a) Overall design of the software; (b) usage flowchart of the software; (c) interface of microscopic image acquisition software; (d) counting hole filling result of cell microscopic image

Fig. 4. Flowchart of cell counting algorithm and cell image processing results. (a1) Original image; (a2) grayscale image; (a3) binary image; (a4) expansion image; (a5) hole filling image; (a6) counting result; (b) flowchart of cell counting algorithm

Fig. 5. Cell migration flowchart and processing results. (a) Cell migration flowchart; (b1) original image; (b2) grayscale image; (b3) edge detection image; (b4) multiple expansion image; (b5) threshold finding image; (b6) delineate area image

Fig. 6. Imaging results by miniaturized microscope under 10 times objective. (a) Rhizome cross-cut of a monocotyledonous plant; (b) onion scale leaves; (c) myocardial longitudinal cut; (d) red blood cells; (e) small intestine section; (f) large intestine section

Fig. 7. Cell migration results after adding mockmock reagent. (a) 0 h; (b) 12 h;(c) 24 h;(d) 36 h;(e) variation in ratio of blank area with time

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