• Laser & Optoelectronics Progress
  • Vol. 62, Issue 2, 0211005 (2025)
Runna Liu*, Jing Liu, Hua Tian, Santing Zheng, and Tingting Zheng
Author Affiliations
  • College of Medicine, Xi'an International University, Xi'an 710077, Shaanxi , China
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    DOI: 10.3788/LOP241029 Cite this Article Set citation alerts
    Runna Liu, Jing Liu, Hua Tian, Santing Zheng, Tingting Zheng. Design of a Miniaturized Cell Microscopic Image Acquisition System Based on Wireless Transmission[J]. Laser & Optoelectronics Progress, 2025, 62(2): 0211005 Copy Citation Text show less
    Design block diagram of miniaturized microscopic image acquisition system based on wireless transmission
    Fig. 1. Design block diagram of miniaturized microscopic image acquisition system based on wireless transmission
    Pictures of miniaturized microscope. (a) Miniaturized microscope system; (b) COMS imaging camera; (c) physical drawing of support and its 3D model; (d) combination diagram of motherboard and objective lens; (e) physical drawing of base and its 3D model; (f) LED array; (g) WiFi module
    Fig. 2. Pictures of miniaturized microscope. (a) Miniaturized microscope system; (b) COMS imaging camera; (c) physical drawing of support and its 3D model; (d) combination diagram of motherboard and objective lens; (e) physical drawing of base and its 3D model; (f) LED array; (g) WiFi module
    Intelligent microscopic image acquisition software. (a) Overall design of the software; (b) usage flowchart of the software; (c) interface of microscopic image acquisition software; (d) counting hole filling result of cell microscopic image
    Fig. 3. Intelligent microscopic image acquisition software. (a) Overall design of the software; (b) usage flowchart of the software; (c) interface of microscopic image acquisition software; (d) counting hole filling result of cell microscopic image
    Flowchart of cell counting algorithm and cell image processing results. (a1) Original image; (a2) grayscale image; (a3) binary image; (a4) expansion image; (a5) hole filling image; (a6) counting result; (b) flowchart of cell counting algorithm
    Fig. 4. Flowchart of cell counting algorithm and cell image processing results. (a1) Original image; (a2) grayscale image; (a3) binary image; (a4) expansion image; (a5) hole filling image; (a6) counting result; (b) flowchart of cell counting algorithm
    Cell migration flowchart and processing results. (a) Cell migration flowchart; (b1) original image; (b2) grayscale image; (b3) edge detection image; (b4) multiple expansion image; (b5) threshold finding image; (b6) delineate area image
    Fig. 5. Cell migration flowchart and processing results. (a) Cell migration flowchart; (b1) original image; (b2) grayscale image; (b3) edge detection image; (b4) multiple expansion image; (b5) threshold finding image; (b6) delineate area image
    Imaging results by miniaturized microscope under 10 times objective. (a) Rhizome cross-cut of a monocotyledonous plant; (b) onion scale leaves; (c) myocardial longitudinal cut; (d) red blood cells; (e) small intestine section; (f) large intestine section
    Fig. 6. Imaging results by miniaturized microscope under 10 times objective. (a) Rhizome cross-cut of a monocotyledonous plant; (b) onion scale leaves; (c) myocardial longitudinal cut; (d) red blood cells; (e) small intestine section; (f) large intestine section
    Cell migration results after adding mockmock reagent. (a) 0 h; (b) 12 h;(c) 24 h;(d) 36 h;(e) variation in ratio of blank area with time
    Fig. 7. Cell migration results after adding mockmock reagent. (a) 0 h; (b) 12 h;(c) 24 h;(d) 36 h;(e) variation in ratio of blank area with time
    Runna Liu, Jing Liu, Hua Tian, Santing Zheng, Tingting Zheng. Design of a Miniaturized Cell Microscopic Image Acquisition System Based on Wireless Transmission[J]. Laser & Optoelectronics Progress, 2025, 62(2): 0211005
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