• Acta Photonica Sinica
  • Vol. 50, Issue 10, 1017002 (2021)
Qi ZENG, Rui LIU, Nan WANG, Tingting ZENG, and Xueli CHEN*
Author Affiliations
  • School of Life Science and Technology,Xidian University,Xi 'an710071,China
  • show less
    DOI: 10.3788/gzxb20215010.1017002 Cite this Article
    Qi ZENG, Rui LIU, Nan WANG, Tingting ZENG, Xueli CHEN. Research Progress of Raman Spectroscopy in Medical Laboratory Science(Invited)[J]. Acta Photonica Sinica, 2021, 50(10): 1017002 Copy Citation Text show less
    Raman scattering electron energy level diagram
    Fig. 1. Raman scattering electron energy level diagram
    Typical laboratory Raman spectroscopy system
    Fig. 2. Typical laboratory Raman spectroscopy system
    Raman spectroscopic apparatus for noninvasive glucose measurement[49]
    Fig. 3. Raman spectroscopic apparatus for noninvasive glucose measurement49
    Raman spectra of Alzheimer's disease patients and healthy people [50]
    Fig. 4. Raman spectra of Alzheimer's disease patients and healthy people 50
    Detection of macroscopic bone by spatial displacement Raman technique[57]
    Fig. 5. Detection of macroscopic bone by spatial displacement Raman technique57
    Treatment methods of liquid samples
    Fig. 6. Treatment methods of liquid samples
    The progress of acetaminophen
    Fig. 7. The progress of acetaminophen
    Spectral pretreatment process
    Fig. 8. Spectral pretreatment process

    Raman related

    technology

    The principleAdvantages & disadvantagesApplicationReference

    The traditional

    Raman

    The frequency of the scattered photon is different from that of the incident photonThe system is relatively simple;low sensitivity

    Drug and food

    testing

    23-25
    Surface Enhanced Raman Scattering(SERS)Use interactions between molecules and coarse metal nanoparticles to enhance Raman signalsSignal enhancement;SERS enhanced reagent is required,which may change the spectrum of endogenous moleculesTumor edge detection26-27
    Coherent anti-Stokes Raman Spectroscopy(CARS)The two beams of incident light illuminate the sample and interact to produce an anti-Stock marketSignal enhancement;the equipment is expensive and has a strong non-resonant backgroundIt is sensitive to C-H bond and lipid detection28-29

    Stimulated Raman Spectroscopy

    (SRS)

    Like CARS,nonlinear interaction between molecules and lasersSignal enhancement,fast imaging;equipment expensiveCell lipid and protein signals were studied by simulated HE staining29-31
    Resonant Raman Spectroscopy(RRS)The excitation wavelength is matched with the molecular electron transition,so the vibration mode is enhancedSignal enhancement,shorten detection time;lasers with continuously adjustable frequencies are requiredNon-destructive detection of pigment molecules such as carotenoids14

    Spatially Offset

    Raman Spectroscopy

    (SORS)

    The scattered light at different depths of the sample produces different shiftsEffectively inhibit fluorescence,provides in-depth organizational information;complex data processingThe application of urinary system,bone,breast and other diseases32-34
    Table 1. Summary and comparison of Raman related techniques
    Qi ZENG, Rui LIU, Nan WANG, Tingting ZENG, Xueli CHEN. Research Progress of Raman Spectroscopy in Medical Laboratory Science(Invited)[J]. Acta Photonica Sinica, 2021, 50(10): 1017002
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