Study on Multi-Wavelength Transmission Spectral Feature Extraction Combined With Support Vector Machine for Bacteria Identification

Chun FENG; Nan-jing ZHAO; Gao-fang YIN; Ting-ting GAN; Xiao-wei CHEN; Min CHEN; Hui HUA; Jing-bo DUAN; Jian-guo LIU

doi:10.3964/j.issn.1000-0593(2021)09-2940-05

Journals >Spectroscopy and Spectral Analysis >Volume 41 >Issue 9 >Page 2940 > Article

Spectroscopy and Spectral Analysis
Vol. 41, Issue 9, 2940 (2021)

Study on Multi-Wavelength Transmission Spectral Feature Extraction Combined With Support Vector Machine for Bacteria Identification

Chun FENG^{1; 2; 3;}, Nan-jing ZHAO^{1; 3; *;}, Gao-fang YIN^{1; 3; *;}, Ting-ting GAN^{1; 3;}, Xiao-wei CHEN^{1; 2; 3;}, Min CHEN^{1; 2; 3;}, Hui HUA^{1; 2; 3;}, Jing-bo DUAN^{1; 3;}, and Jian-guo LIU^{1; 3;}

Author Affiliations

1. Key Laboratory of Environmental Optics and Technology, Anhui Institute of Optics and Fine Mechanics, Chinese Academy of Sciences, Hefei 230031, China

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DOI: 10.3964/j.issn.1000-0593(2021)09-2940-05 Cite this Article

Chun FENG, Nan-jing ZHAO, Gao-fang YIN, Ting-ting GAN, Xiao-wei CHEN, Min CHEN, Hui HUA, Jing-bo DUAN, Jian-guo LIU. Study on Multi-Wavelength Transmission Spectral Feature Extraction Combined With Support Vector Machine for Bacteria Identification[J]. Spectroscopy and Spectral Analysis, 2021, 41(9): 2940 Copy Citation Text

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Multi-wavelength transmission spectra and normalized images of S.aureus at different concentrations(a): Spectra of S.aureus at different concentrations; (b): Normalized spectra of different concentrations;(c): Normalized spectra at high concentrations; (d): Normalized spectra at low concentrations

Fig. 1. Multi-wavelength transmission spectra and normalized images of S.aureus at different concentrations
(a): Spectra of S.aureus at different concentrations; (b): Normalized spectra of different concentrations;(c): Normalized spectra at high concentrations; (d): Normalized spectra at low concentrations

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Fig. 2. Normalized trend graphs of multi-wavelength transmission spectra of five low bacteria concentration samples

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Fig. 3. Variance analysis diagram of multi-wavelength transmission spectrum of bacteria

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1 E.coli 2 K.pneumoniae 3 S.aureus 4 S.typhi 5 P.aeruginosa
A original B normalized
	1-2	1-3	1-4	1-5	2-3	2-4	2-5	3-4	3-5	4-5
A	0.999 4	0.964 0	0.997 9	0.988 2	0.967 0	0.997 2	0.985 2	0.946 5	0.915 2	0.995 0
B	0.999 4	0.964 0	0.997 9	0.988 2	0.967 0	0.997 2	0.985 2	0.946 5	0.915 2	0.995 0

Table 1. Cosine similarity between different microbial spectra of bacteria

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Bacteria	Optical density/a.u. (200 nm)	Slope 1 (200~245 mm)	Slope 2 (250~275 mm)	Slope 3 (280~300 mm)
K. Pneumoniae	0.006 5	-62.45	-15.48	-29.96
S. Aureus	0.005 1	-35.94	-14.82	-24.62
S. Typhi	0.007 4	-81.30	-20.91	-33.71
E. Coli	0.007 5	-82.67	-13.92	-36.09
P.Aeruginosa	0.008 5	-103.49	-26.21	-30.88

Table 2. The difference of normalized graph of multi-wavelength transmission spectra of five kinds of bacteria

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Bacteria	Train set	Predict set	recognition rate/%
K. Pneumoniae	9	9	100.0
S. Aureus	8	7	100.0
S. Typhi	10	10	100.0
E. Coli	13	13	100.0
P. Aeruginosa	9	8	100.0
-	49	47	100.0

Table 3. The recognition rates of five kinds of bacteria by libsvm

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