Wind profile radar is a new kind of instruments in remote sensing sounding, the echo signals of which contain abundant informations of turbulence. An algorithm for retrieval dynamic turbulence is proposed using the echo of wind profile radar, and the characteristic of velocity structure constant C2v in troposphere is analyzed. Also, the turbulent velocity fluctuation in troposphere is discussed based on the velocity turbulence model. The results show that: 1) the strength of dynamic turbulence decreases along with the increase of the height, the lapse rate of which in the night is more rapid, and the C2v has remarkable diurnal variability at the top of boundary layer; 2) there is good linear relation between C2v and the natural logarithm of velocity fluctuation variance σ2, and the closeness of linear fitting could reach 0.896; 3) the velocity fluctuation of turbulence in troposphere is relatively small, and σ2 distributes between 10-3 m2·s-2 and 10-2 m2·s-2 in clear-air.