Human eyes′ contrast sensitivity function (CSF) at temporal frequencies and wave-front aberrations of optical system of the eye have been measured respectively, and then the neural contrast sensitivity function (NCSF) is calculated by the correlation between them. The results demonstrate that the overall value of NCSF decreases as the temporal frequency increases. From the low to the high temporal frequencies (the testing temporal frequencies of 1~30 cycle/s for two eyes and 1~24 cycle/s for the other two), the relative decreasing amounts of NCSF are: 90%, 87%, 60% and 68%, respectively. Comparing with the corresponding CSF at temporal frequencies, the NCSF curve is smooth at low and middle spatial frequencies [2~10 cycle/(°)] and decreases appreciably at high spatial frequencies [greater than 10 cycle/(°)]. The values of NCSF curves at the same temporal frequency for 4 subjects′ eyes are approximate, which demonstrates that the responding of the human eyes (without optic nerve disease) to the same temporal frequency is identical.