Far Ultraviolet wavelengths (FUV 115 to 200 nm) optical remote sensing detection on satellite is one of the most promising technologies on space weather. This technology can be effectively used to obtain some important space environment parameters, such as O, N2, O2 column density and profile distribution, total electron content (TEC) of ionosphere and electron density profile. It also yields valuable information on content of plasma, temperature profile of the atmosphere, solar EUV flux and energy particle. In the processes of obtaining these physical parameters quantitatively there is one key step—the radiation calibration, including pre-launch laboratory calibration and on-orbit calibration: the former gives original calibration coefficient of the load before launch; the latter checks the change of the calibration coefficient of the instrument’s on-orbit performance after a set period. In the West, FUV technology application to upper atmosphere, ionosphere, magnetosphere and solar activity started in the 1970s, and has been applied to the long-term Space Weather Program Strategic Plan by U. S. federal government. In China, this technology was carried out at the beginning of the 21st century, and the in-flight calibration is still a research gap now. This paper introduces on-orbit calibration technologies for some representative far ultraviolet payload based on the external standard radiation source, internal radiation standard source and vicarious calibration, and then analyses the data processing method and the results on three kinds of FUV remote sensing: Imager, Spectrometer Imager and Photometer. The results suggest that the best on-orbit calibration method for imager or spectrometer imager is using UV stellar as an external radiation standard; the instrument can catch the known radiation from the stellar; using the radiation from the stellar and the spectral responsibility from the lab, the sensitivity of the instrument will be known as in-flight. The internal standard radiation source, like deuterium lamp, is not a good choice for in-flight calibration becauseradiation itself from lamp attenuates. On single-point detection instrument, like a photometer with limited visual field, the vicarious calibration is a good way to realize on-orbit calibration. During the process of calibration, data selecting and spatio-temporal matching should be cautiously conducted in order to enhance the calibration accuracy.