Tb³⁺ can form chelate with pazufloxacin mesylas (PM),and the complex of terbium-pazufloxacin mesylas(Tb³⁺ -PM) can sensitize the chemiluminescence (CL) intensity of KMnO₄-Na₂SO₃ system through energy transfer.Based on the chemiluminescence reaction,a new sensitized chemiluminescence method was developed for the determination of PM by flow-injection.Under the optimum conditions,the enhanced CL intensity was linear with the concentration of PM in the range of 0.10-12 mg·L^-1 with the detection limit of 0.04 mg·L^-1 .The relative standard deviation for eleven continuous determinations of 1.0 mg·L^-1 PM was 1.9%.This method has been applied to the determination of PM in injection and body fluid with satisfactory results.This method has the merits of simpleness,speediness,sensitivity and good reproducibility.An original mechanism was proposed to explain this phenomenon.