OCT combined with reflective spectrum was employed to precisely evaluate the mice scald model.Under the conditions that the temperature increased by 5 ℃ from 60 to 95 ℃ with 20 s thermal effect, the cutis depth linearly increased.The fitting absorption parameters and the gradients showed the V trends.Wave peaks of first order differential were legible from 65 to 85 ℃, then wave peaks became worse over 85 ℃.At color coordinates, red value decreased severely from 65 to 85 ℃ and from 90 to 95 ℃, green value continuously decreased, but blue value increased.Under the conditions that thermal effect time increased from 10 to 40 s at 90 ℃, the main changes of above factors happened at about 20 s, and after 20 s effect time the change of these factors was weak.These factors could reflect the form and progresses of zones of coagulation, injury of cutis and hypodermis.So OCT combined with reflection spectrum could provide a novel method that can be applied for the real-time, low-cost, in vivo and noninvasive optical biopsy on scald.