The spectrally tunable source based on spatial light modulator (SLM) is a novel source applying to remote sensor calibration, which aimed at achieving a new calibration model about spectral matching between calibration source and observed scene to further improve spectral and radiometric calibration accuracy. According to the non-photoconductive characteristic of the digital micromirror device and the special operating modes of the source, a spectral calibration scheme based on laser wavelengths and field spectroradiometer was adopted for coarse and precise spectral calibration, and a temporary radiometric calibration method based on lamp-plaque system and field spectroradiometer was adopted. Whereafter, this source was calibrated at 470～945 nm band spectrally and radiometrically. The uncertainty analysis showed that the accuracy of spectral calibration was better than 0.2 nm, and the combined radiometric calibration uncertainty was less than 3.1 % (k=1).