Image processing for nanoparticle sizing with dynamic light scattering (DLS) technique is introduced. A CCD is used to capture the spatial distribution of the scattered light by nanoparticles under Brownian motion. The sizes of the nanoparticles can be determined with the post-processing of the recorded data by calculating the autocorrelation functions of the scattered light. Standard nanoparticles of four different sizes (27, 79, 482, 948 nm) are used in the experiments. Since the frame rate of CCD is far lower than the frequency of photomultiplier, 55% glycerol/water emulsion is employed as disperse medium here, therefore. The measurement deviation is dramatically reduced from 15.1% to 1.9% in 27 nm nanoparticles at frame rate of 8290 frame/s. Compared with conventional DLS, this technique is advantageous due to the compact and simple measurement system with short measurement time which is 1% of conventional DLS measurement time.