Based on the self-assembly experimental installation, the influence of flexoelectric effect on the dynamic response of strong anchoring hybrid aligned nematic (HAN) liquid crystal is studied. The positive and negative monopulse with pulse width of 250 ms and adjustable amplitude are applied to HAN cell during experiment. The results show that the dynamic response of liquid crystal is different from the pulse with the same amplitude, as well as from the positive pulse or negative pulse with different amplitudes. Through comparing the experimental curves of the dynamic response of liquid crystal for different positive pulses 3 V, 4 V, 5 V, 6 V with the corresponding theoretical curves with the flexoelectric effect considered, the sum of flexoelectric coefficient of liquid crystal material E7 is e11+e33=4.0×10-11 C/m, which is in agreement with the experimental measurement value reported in the literature.