Currently, there are some problems on indoor test methods relating to low contrast dynamic target acquisition in photoelectric tracker, and related theory about low contrast dynamic target acquisition. Because low contrast dynamic target acquisition is one of the important factors relating to overall performance of photoelectric tracker, a new indoor method for performance assessment of low contrast dynamic target acquisition in photoelectric tracker is proposed. Infinite target source of adjustable contrast and turntable of adjustable speed are considered as detection device. Optical reference target, of which the contrast can be accurately measured, is provided for detecting opto-electronic tracker working in dynamic environments where the rotation rate can be precisely set, in order to validate acquisition ability of opto-electronic tracker for low contrast dynamic object based on target extraction. The opto-electronic tracker can also be evaluated in terms of acquisition performance or acquisition speed in different target contrasts and different movement speeds by the analyses of the target′s movement speed, target size that affects target contrast. The experimental results show that the photo-electronic tracker cannot effectively capture the object of which the diameter is 4 mm entering the field of view at the rate of greater than 7.45°/s from infinity in the background of which the spectral radiance is 38 W/(sr·m2). Furthermore, the results can be applied to evaluate the acquisition ability of photo-electric tracker by a given contrast dynamic target.