• Journal of Resources and Ecology
  • Vol. 11, Issue 2, 129 (2020)
Wei DU, Yue LI, Pei HE, Jiaqi ZHANG, Haichao JING, Cheng NIE, and Yinghui LIU
Author Affiliations
  • State Key Laboratory of Earth Surface Processes and Resource Ecology, Faculty of Geographical Science, Beijing Normal University, Beijing 100875, China
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    DOI: 10.5814/j.issn.1674-764x.2020.02.001 Cite this Article
    Wei DU, Yue LI, Pei HE, Jiaqi ZHANG, Haichao JING, Cheng NIE, Yinghui LIU. Nitrogen Addition Decreases Soil Respiration without Changing the Temperature Sensitivity in a Semiarid Grassland[J]. Journal of Resources and Ecology, 2020, 11(2): 129 Copy Citation Text show less
    Dynamics of soil temperature and soil moisture for 0-20 cm surface soil and precipitation during the growing seasons of 2013 (a), 2014 (b), and 2015 (c).
    Fig. 1. Dynamics of soil temperature and soil moisture for 0-20 cm surface soil and precipitation during the growing seasons of 2013 (a), 2014 (b), and 2015 (c).
    Soil variables with six N gradients and three years (n=3) Note: (a) Dissolved organic carbon; (b) Inorganic nitrogen; (c) Ph; (d) Total organic carbon; (e) Total nitrogen; (f) Aboveground biomass; (g) PLFAs; (h) Fungi to bacteria ratio; (i) Gram-positive bacteria to gram-negative bacteria ratio; (j) Soil respiration; (k) Q10.
    Fig. 2. Soil variables with six N gradients and three years (n=3) Note: (a) Dissolved organic carbon; (b) Inorganic nitrogen; (c) Ph; (d) Total organic carbon; (e) Total nitrogen; (f) Aboveground biomass; (g) PLFAs; (h) Fungi to bacteria ratio; (i) Gram-positive bacteria to gram-negative bacteria ratio; (j) Soil respiration; (k) Q10.
    Principal component analysis of microbial community composition in soil samples with six N gradients and three years (mean±SE) (n=3). Soil samples in the three years are presented by different colors, as black indicates 2013, white indicates 2014, and grey indicates 2015.
    Fig. 3. Principal component analysis of microbial community composition in soil samples with six N gradients and three years (mean±SE) (n=3). Soil samples in the three years are presented by different colors, as black indicates 2013, white indicates 2014, and grey indicates 2015.
    Structural equation model for the effects of nitrogen addition, soil temperature and soil moisture on soil pH, C and N availability, aboveground biomass, total PLFAs, microbial composition, Rs and Q10. Note: Dashed, red, and blue paths indicate the effect is insignificant, positive, and negative, respectively. The width of the arrow indicates the magnitude of the standardized path coefficient. Bold and italic-typed values indicate the proportions of the variance explained for each variable. χ2—chi-square, df—degrees of freedom, P— P value, RMSEA—root mean square error of approximation, CFI—comparative fit index.
    Fig. 4. Structural equation model for the effects of nitrogen addition, soil temperature and soil moisture on soil pH, C and N availability, aboveground biomass, total PLFAs, microbial composition, Rs and Q10. Note: Dashed, red, and blue paths indicate the effect is insignificant, positive, and negative, respectively. The width of the arrow indicates the magnitude of the standardized path coefficient. Bold and italic-typed values indicate the proportions of the variance explained for each variable. χ2—chi-square, df—degrees of freedom, PP value, RMSEA—root mean square error of approximation, CFI—comparative fit index.
    FactorPC1
    PLFA biomarkers
    i14:00.050
    i15:0-0.358
    a15:0-0.687
    i16:0-0.572
    a16:0-0.718
    16:1 w7c0.055
    16:1 w5c0.699
    16:00.108
    i17:0-0.551
    a17:0-0.492
    17:1 w8c0.216
    cy17:0-0.474
    10Me17:00.377
    18:2 w6,9c0.679
    18:1 w9c0.679
    18:1 w7c0.412
    18:1 w5c0.866
    18:00.685
    10Me18:00.701
    C and N availability
    DOC0.824
    TOC0.872
    TN0.949
    Table 1.

    Loadings of microbial compositions and C and N availability with PC1 in principal component analysis on soil samples with six N addition levels and three years

    NSTSMpHDOCINTOCTNABMPLFAsF:BG+:G-RsQ10
    N1
    ST0.1651
    SM-0.083-0.308*1
    pH-0.861**-0.038-0.0131
    DOC0.352**0.277*-0.365**-0.277*1
    IN0.729**0.126-0.100-0.690**0.2091
    TOC-0.1370.309*-0.2600.1740.501**-0.1431
    TN0.0310.260-0.2670.0430.702**-0.0990.795**1
    ABM-0.0410.136-0.1150.0640.0710.1380.081-0.1011
    PLFAs-0.298*-0.0910.395**0.366**-0.124-0.493**0.0960.220-0.452**1
    F:B-0.504**-0.124-0.0390.417**-0.469**-0.138-0.202-0.500**0.378**-0.432**1
    G+:G-0.455**-0.1470.152-0.472**0.1160.280*-0.0800.188-0.2590.153-0.543**1
    Rs-0.271*0.1370.510**0.323*-0.366**-0.334*-0.068-0.059-0.1790.608**-0.1190.0391
    Q10-0.089-0.309*0.718**0.058-0.358**-0.043-0.243-0.273*-0.0110.2550.0300.0160.436**1
    Table 2.

    Pearson correlation analysis of soil respiration and the impact factors

    FactorNSTSMpHCNABMPLFAsMicrobial composition
    ABM-0.070.13-0.080.04-0.06N/AN/AN/A
    PLFAs-0.27-0.030.370.550.25-0.44N/AN/A
    Microbial composition-0.610.10-0.300.19-0.210.39N/AN/A
    Rs(a)-0.270.330.560.40-0.09-0.180.42N/A
    Q10(a)-0.02-0.080.710.02-0.080.080.04N/A
    Rs(b)-0.250.330.560.47-0.09-0.18N/A-0.36
    Q10(b)-0.04-0.080.710.01-0.080.08N/A0.05
    Table 3.

    Standardized total effects in structural equation models

    Wei DU, Yue LI, Pei HE, Jiaqi ZHANG, Haichao JING, Cheng NIE, Yinghui LIU. Nitrogen Addition Decreases Soil Respiration without Changing the Temperature Sensitivity in a Semiarid Grassland[J]. Journal of Resources and Ecology, 2020, 11(2): 129
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