Fourier transform spectroscopy (FTIR) and two-dimensional correlationspectroscopy were used to analyze different compatibility forms ofsargentgloryvine stem, radix paeoniae rubra and cortex moutan in order to studythe FTIR spectra of different proportion formulas. The results indicate thatdifferent proportion formulas have distinct change regularity in FTIR spectra,second derivative spectra and synchronous 2D. Key components of sargentgloryvinestem’s characteristic absorption band are 1 610, 1 518 and 1 446 cm-1 which arecharacteristic absorption band of aromatic material, and in the original formula,that shows stronger peak than others, suggesting that original formula can makethe best of composition of drug action. In the different proportion formulas, 1614 cm-1 is nearer to 1 610 cm-1, which is sargentgloryvine stem’scharacteristic absorption band, than to 1 706 cm-1, illustrating thatsargentgloryvine stem is more influential formula than others. According toidentification and ascription of characteristic absorption band, it was initiallyrevealed that different proportion of drug dosage can affect pharmacodynamicaction of integral formula.