Rui-rui ZHANG, Wei-qian ZHAO, Yun WANG, Li-rong QIU. High-Stability Confocal Raman Microscopy Imaging Method Based on Bilateral Fitting Subtracting Confocal Microscopy[J]. Spectroscopy and Spectral Analysis, 2021, 41(9): 2795
- Spectroscopy and Spectral Analysis
- Vol. 41, Issue 9, 2795 (2021)
Fig. 1. Diagram of confocal Raman microscopy
Fig. 2. The relationship between the lateral FWHM and the defocusing amount u
Fig. 3. (a) Influence of axial scanning pitch on RMSE at different spherical aberration; (b) Influence of axial scanning pitch on RMSE at different astigmatism; (c) Influence of axial scanning pitch on RMSE at different SNR
Fig. 4. Actual axial response curve and focusing result of BFSCRM
C I is the actual curve; l L and l R are fitting lines; l D is difference line; z f=199.695 μm is the focus position
Fig. 5. Focusing repetition accuracy of BFSCRM
Fig. 6. (a) microscopic observation image; (b) zoom-in the area shown in red area of (a); (c) Raman spectrum of point A in (a)
Fig. 7. Raman images of a vertical bar with a 5 μm period
(a): Without auto-focusing; (b): Using BFSCRM
(a): Without auto-focusing; (b): Using BFSCRM
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