• Chinese Journal of Lasers
  • Vol. 47, Issue 2, 207004 (2020)
Han Tao, Qiu Jianrong, Wang Di, Meng Jia, Liu Zhiyi, and Ding Zhihua*
Author Affiliations
  • State Key Laboratory of Modern Optical Instrumentation, College of Optical Science and Engineering,Zhejiang University, Hangzhou, Zhejiang 310027, China
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    DOI: 10.3788/CJL202047.0207004 Cite this Article Set citation alerts
    Han Tao, Qiu Jianrong, Wang Di, Meng Jia, Liu Zhiyi, Ding Zhihua. Optical Coherence Microscopy and Its Application[J]. Chinese Journal of Lasers, 2020, 47(2): 207004 Copy Citation Text show less
    Diagram of TD-OCM system and axial PSF[23]. (a) Schematic of TD-OCM system; (b) comparison of axial PSF under confocal and coherence-gated configuration of OCM system
    Fig. 1. Diagram of TD-OCM system and axial PSF[23]. (a) Schematic of TD-OCM system; (b) comparison of axial PSF under confocal and coherence-gated configuration of OCM system
    Schematic of FD-OCM system and comparison of sensitivity between TD-OCM and FD-OCM. (a) Schematic of FD-OCM system; (b) comparison of sensitivity between TD-OCM and FD-OCM with Gaussian source at 1300 nm[27]
    Fig. 2. Schematic of FD-OCM system and comparison of sensitivity between TD-OCM and FD-OCM. (a) Schematic of FD-OCM system; (b) comparison of sensitivity between TD-OCM and FD-OCM with Gaussian source at 1300 nm[27]
    Overview of FF-OCM system
    Fig. 3. Overview of FF-OCM system
    Principle of data acquisition in FF-OCM system
    Fig. 4. Principle of data acquisition in FF-OCM system
    Illustrations of scanning imaging modes of PS-OCM and FF-OCM. (a) Scanning imaging mode of PS-OCM; (b) scanning imaging mode of FF-OCM
    Fig. 5. Illustrations of scanning imaging modes of PS-OCM and FF-OCM. (a) Scanning imaging mode of PS-OCM; (b) scanning imaging mode of FF-OCM
    Simulation of resolution varying with source central wavelength, bandwidth, and numerical aperture of objective in OCM. (a) Relation among axial resolution, source central wavelength, and bandwidth; (b) relation among lateral resolution, source central wavelength, and numerical aperture
    Fig. 6. Simulation of resolution varying with source central wavelength, bandwidth, and numerical aperture of objective in OCM. (a) Relation among axial resolution, source central wavelength, and bandwidth; (b) relation among lateral resolution, source central wavelength, and numerical aperture
    Ultrahigh resolution images of industrial sandpaper[38]. (a) Industrial sandpaper with particle size about 125 μm; (b) sandpaper longitudinal section image of general system; (c) sandpaper cross section image of ultra-high resolution system
    Fig. 7. Ultrahigh resolution images of industrial sandpaper[38]. (a) Industrial sandpaper with particle size about 125 μm; (b) sandpaper longitudinal section image of general system; (c) sandpaper cross section image of ultra-high resolution system
    High-resolution images of onion epithelium obtained by FF-OCM system[29]
    Fig. 8. High-resolution images of onion epithelium obtained by FF-OCM system[29]
    Images of scattering samples without and with random phase[49].(a) Without random phase; (b) with random phase
    Fig. 9. Images of scattering samples without and with random phase[49].(a) Without random phase; (b) with random phase
    Axial resolution of OCM system versus detection depth and source bandwidth[13]. (a) Axial resolution versus FWHM of the Gaussian spectrum at different depths of human epidermis; (b) reachable minimum axial resolution versus depth of epidermis
    Fig. 10. Axial resolution of OCM system versus detection depth and source bandwidth[13]. (a) Axial resolution versus FWHM of the Gaussian spectrum at different depths of human epidermis; (b) reachable minimum axial resolution versus depth of epidermis
    Diagram of dark-field OCM with extended depth of focus in sample arm
    Fig. 11. Diagram of dark-field OCM with extended depth of focus in sample arm
    En face images of murine pancreas at different depths obtained by extended-focus OCM system[68]. Scale bar: 200 μm. (a) 11 μm; (b) 54 μm; (c) 97 μm; (d) 110 μm
    Fig. 12. En face images of murine pancreas at different depths obtained by extended-focus OCM system[68]. Scale bar: 200 μm. (a) 11 μm; (b) 54 μm; (c) 97 μm; (d) 110 μm
    Example of imaging process of human fingertip by GD-OCM[71]
    Fig. 13. Example of imaging process of human fingertip by GD-OCM[71]
    Cross-section images of African frog tadpole[70]. (a) Image acquired by GD-OCM system; (b) image acquired by OCM system at fixed focal plane
    Fig. 14. Cross-section images of African frog tadpole[70]. (a) Image acquired by GD-OCM system; (b) image acquired by OCM system at fixed focal plane
    Images of posterior layers of human corneas of healthy and FED corneas[91]. (a)(c)(d) Images of healthy cornea in the posterior elastic layer, endothelial cell layer, and posterior stromal layer; (b)(e)(f) images of FED cornea in the posterior elastic layer, endothelial cell layer, and posterior stromal layer
    Fig. 15. Images of posterior layers of human corneas of healthy and FED corneas[91]. (a)(c)(d) Images of healthy cornea in the posterior elastic layer, endothelial cell layer, and posterior stromal layer; (b)(e)(f) images of FED cornea in the posterior elastic layer, endothelial cell layer, and posterior stromal layer
    Ex vivo images of cortical and subcortical structures in mouse brain slice[39]. (a) Transmission image showing imaging area; (b) result of Vis-OCM imaging; (c)(d) Vis-OCM images, fluorescence images of labeled amyloid plaques, and overlays of cortical and subcortical structures
    Fig. 16. Ex vivo images of cortical and subcortical structures in mouse brain slice[39]. (a) Transmission image showing imaging area; (b) result of Vis-OCM imaging; (c)(d) Vis-OCM images, fluorescence images of labeled amyloid plaques, and overlays of cortical and subcortical structures
    En face images of fibroblast obtained by dynamic detection[110]. Scale bar: 50 μm.(a) En face image; (b) fiber stretch detection; (c) cell migration detection
    Fig. 17. En face images of fibroblast obtained by dynamic detection[110]. Scale bar: 50 μm.(a) En face image; (b) fiber stretch detection; (c) cell migration detection
    Diagram of focus-extended OCM and angiograms of mouse cortex[109]. (a) Illustration of focus-extended system; (b)(c) interpolation process for angiogram; (d) angiogram of mouse cortex; (e)(f) quantitative distributions of total flow rate and axial flow rate
    Fig. 18. Diagram of focus-extended OCM and angiograms of mouse cortex[109]. (a) Illustration of focus-extended system; (b)(c) interpolation process for angiogram; (d) angiogram of mouse cortex; (e)(f) quantitative distributions of total flow rate and axial flow rate
    CategoryCSLMOCTPS-OCMFF-OCM
    Axial resolution δz /μm0.5--20.5--80.5--80.5--8
    Lateral resolution δx /μm0.2--110--200.5--20.5--2
    Imaging depth zm /mm<0.21--3<0.2
    Field of view xm /mm2200--400>1000200--400200--400
    Imaging speed /(pixel·s-1)<5×107>1×1010>1×1010(2--5)×109
    Power P /μW~110--10010--1000.5--2
    Sensitivity RSN /dB<40100--110100--11080--90
    Table 1. Typical performance parameters of different imaging systems[88-90]
    Han Tao, Qiu Jianrong, Wang Di, Meng Jia, Liu Zhiyi, Ding Zhihua. Optical Coherence Microscopy and Its Application[J]. Chinese Journal of Lasers, 2020, 47(2): 207004
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