A novel enhanced ultraviolet absorption spectrometry method was developed for the quantitative analysis of SDBS induced by β-cyclodextrin(β-CD) with strong interferences. The ultraviolet absorption spectra of SDBS indicated that the presence of β-CD could result in the enhancement of absorption intensities of SDBS. A good linearity was obtained between the UV-absorption intensity of the system and the concentration of SDBS. The results indicated that the determination precision and the determination ranges of SDBS could be greatly improved by β-CD. The effect of several common interfering substances(SDS, OP-10, HPAM) on the determination of SDBS could be significantly reduced in β-CD aqueous solution. Therefore, the maximum errors of the determined SDBS were less than 2.0% under multifactor interferences, and the precision of the method was also as high as 10－2～10－3 mg·L-1. The stable inclusion of β-CD and SDBS could be automatically formed in water with molar ratio of 1∶1. The stability constant of the inclusion, Ka, was 87 and the standard Gibbs function of molar reaction, ΔγGmΘ(298 K), was －11.064 kJ·mol－1. FTIR analysis exhibited that SDBS could be induced by β-CD since the phenyl group in SDBS molecule could exist stably in the cavity of β-CD and form the inclusion.