• Spectroscopy and Spectral Analysis
  • Vol. 41, Issue 9, 2770 (2021)
Juan-juan FU*, Dan-ying MA, Jin-lan TANG, Yi-lin BAO, Yuan ZHAO, Lin-wei SHANG, and Jian-hua YIN*;
Author Affiliations
  • Department of Biomedical Engineering, Nanjing University of Aeronautics and Astronautics, Nanjing 211106, China
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    DOI: 10.3964/j.issn.1000-0593(2021)09-2770-06 Cite this Article
    Juan-juan FU, Dan-ying MA, Jin-lan TANG, Yi-lin BAO, Yuan ZHAO, Lin-wei SHANG, Jian-hua YIN. NIR Spectroscopic Study and Staging Diagnosis of Osteoarthritic Articular Cartilage[J]. Spectroscopy and Spectral Analysis, 2021, 41(9): 2770 Copy Citation Text show less
    The near-infrared average spectra of healthy and multi-stage OA tissues at SZ(a), TZ(b), DZ(c), respectively
    Fig. 1. The near-infrared average spectra of healthy and multi-stage OA tissues at SZ(a), TZ(b), DZ(c), respectively
    The absorbance ratios of the 4 610 to 4 898 cm-1 band (a), the complex to 4 898 cm-1 band (b), the complex to 4 610 cm-1 band (c) of healthy and multi-stage OA tissues at different depths (SZ, TZ, DZ), respectively
    Fig. 2. The absorbance ratios of the 4 610 to 4 898 cm-1 band (a), the complex to 4 898 cm-1 band (b), the complex to 4 610 cm-1 band (c) of healthy and multi-stage OA tissues at different depths (SZ, TZ, DZ), respectively
    The scatter plots of the first 3 principal factors on the SZ NIR spectra of healthy and OA tissues with preprocess of BC(a), 1st-2-21SG (b) and 2nd-3-25SG (c), respectively
    Fig. 3. The scatter plots of the first 3 principal factors on the SZ NIR spectra of healthy and OA tissues with preprocess of BC(a), 1st-2-21SG (b) and 2nd-3-25SG (c), respectively
    分组实验犬
    编号
    软骨切
    块编号
    切片深度
    /μm
    切片数量
    /个
    OA-3MY1150~80016
    250~70014
    Y23100~1 05020
    41001
    Y3550~55011
    650~70014
    OA-7MY4750~95019
    850~95019
    Y5950~90018
    Y61050~70014
    111001
    健康Y71250~85017
    1350~1 05021
    1450~60012
    Y81550~4509
    16501
    17501
    Y91850~50010
    Table 1. Sample information of horizontal section of articular cartilage
    预处理方式SZ识别
    率/%
    TZ识别
    率/%
    DZ识别
    率/%
    BC8076.271.3
    初始案例1st-2-21SG9585.773.8
    2nd-3-25SG8576.255.7
    BC5071.467.2
    交叉验证1st-2-21SG9085.771.8
    2nd-3-25SG7042.954.0
    Table 2. Fisher discrimination of healthy and OA tissues with different pretreatment methods of NIR spectroscopy
    组别主因子数PC/个Wilk's lambdasig.
    SZ50.3450.006
    TZ50.3660.005
    DZ50.7720.000
    Table 3. PCA-FDA significant test results of NIR spectral data at SZ, TZ and DZ with preprocess of 1st-2-21SG
    类别光谱数据初始判别结果交叉判别结果识别率/%
    健康组OA-3MOA-7M健康组OA-3MOA-7M初始案例交叉验证
    健康组666
    训练集OA-3M54113193.887.5
    OA-7M555
    健康组33
    预测集OA-3M21187.5
    OA-7M33
    Table 4. PCA-FDA of NIR spectral training and prediction sets of healthy and multi-stage OA cartilage (SZ, TZ, DZ)
    Juan-juan FU, Dan-ying MA, Jin-lan TANG, Yi-lin BAO, Yuan ZHAO, Lin-wei SHANG, Jian-hua YIN. NIR Spectroscopic Study and Staging Diagnosis of Osteoarthritic Articular Cartilage[J]. Spectroscopy and Spectral Analysis, 2021, 41(9): 2770
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