Identifying Bloodstain Species Using Hyperspectral Imaging

Yuan Zhuang; Shuhui Gao; Fei Xie; Wei Huang; Nengbin Cai

doi:10.3788/LOP202259.1630001

Journals >Laser & Optoelectronics Progress >Volume 59 >Issue 16 >Page 1630001 > Article

Laser & Optoelectronics Progress
Vol. 59, Issue 16, 1630001 (2022)

Identifying Bloodstain Species Using Hyperspectral Imaging

Yuan Zhuang¹, Shuhui Gao^1、*, Fei Xie¹, Wei Huang², and Nengbin Cai³

Author Affiliations

¹School of Investigation, People’s Public Security University of China, Beijing 100038, China

²Institute of Forensic Science, Ministry of Public Security, Beijing 100038, China

³Shanghai Key Laboratory of Crime Scene Evidence, Shanghai 200083, China

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DOI: 10.3788/LOP202259.1630001 Cite this Article Set citation alerts

Yuan Zhuang, Shuhui Gao, Fei Xie, Wei Huang, Nengbin Cai. Identifying Bloodstain Species Using Hyperspectral Imaging[J]. Laser & Optoelectronics Progress, 2022, 59(16): 1630001 Copy Citation Text

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Fig. 1. Workflow of imaging spectrometer

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Fig. 2. Chicken blood and bovine blood used in experiment. (a) Chicken blood; (b) bovine blood

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Fig. 3. Spectral curves of human blood, chicken blood, and mixed human and chicken blood on five carriers

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Fig. 4. Spectral curves of human blood, bovine blood, and mixed human and bovine blood on five carriers

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Fig. 5. Differentiation of three comparative samples on 30 vectors

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Fig. 6. Differentiation of three comparative samples on 30 vectors

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Number	Carrier	Color	Number	Carrier	Color
1#	Canvas	Dard Red	16#	Leather	Black
2#	Canvas	White	17#	Leather	Brown
3#	Canvas	Bright Red	18#	Leather	Green
4#	Canvas	Black	19#	Chamois	Dard Red
5#	Canvas	Brown	20#	Chamois	White
6#	Canvas	Green	21#	Chamois	Bright Red
7#	Cotton	Dard Red	22#	Chamois	Black
8#	Cotton	White	23#	Chamois	Brown
9#	Cotton	Bright Red	24#	Chamois	Green
10#	Cotton	Black	25#	Paperboard	Pink
11#	Cotton	Brown	26#	Paperboard	Bright Red
12#	Cotton	Green	27#	Paperboard	Yellow
13#	Leather	Dard Red	28#	Paperboard	Blue
14#	Leather	White	29#	Invoice	White
15#	Leather	Bright Red	30#	Envelope	Brown

Table 1. Information and serial number of carriers used in experiment

Degree	Difference of rank mean（a）	Result of assessment
1	a≤15	-
2	15<a≤30	+
3	a>30	++

Table 2. Basis of quality assessment

Number	Carrier	Human blood and chicken blood	Human blood and the mix of human and chicken blood	Chicken blood and the mix of human and chicken blood
1	Dark red canvas	10.84	9.16	1.44
2	Brown cotton	40.60	37.36	37.50
3	Bright red chamois	35.36	34.18	25.04
4	Yellow paperboard	27.90	21.24	18.76
5	Invoice	49.00	32.52	29.44

Table 3. Rank mean difference of human blood, chicken blood, and mixed blood of human and chicken on five vectors

Number	Carrier	Human blood and chicken blood	Human blood and the mix of human and chicken blood	Chicken blood and the mix of human and chicken blood
1	Dark red canvas	-	-	-
2	Brown cotton	++	++	++
3	Bright red chamois	++	++	+
4	Yellow paperboard	+	+	+
5	Invoice	++	++	+

Table 4. Quality assessment results of human blood, chicken blood, and mixed human and chicken blood on five carriers

Number	Carrier	Human blood and bovine blood	Human blood and the mix of human and bovine blood	Bovine blood and the mix of human and bovine blood
1	Bright red canvas	43.70	30.14	24.10
2	Black cotton	33.44	13.04	34.30
3	Green leather	39.78	25.74	22.72
4	Bright red chamois	43.50	35.28	26.26
5	Envelope	37.68	27.62	24.84

Table 5. Rank mean difference of human blood, bovine blood, and mixed human and bovine blood on five vectors

Number	Carrier	Human blood and bovine blood	Human blood and the mix of human and bovine blood	Bovine blood and the mix of human and bovine blood
1	Bright red canvas	++	++	+
2	Black cotton	++	-	++
3	Green leather	++	+	+
4	Bright red chamois	++	++	+
5	Envelope	++	+	+

Table 6. Quality evaluation results of human blood, bovine blood, and mixed human and bovine blood on five vectors

Number	Human blood and chicken blood	Human blood and the mix of human and chicken blood	Chicken blood and the mix of human and chicken blood	Human blood and bovine blood	Human blood and the mix of human and bovine blood	Bovine blood and the mix of human and bovine blood
1#	-	-	-	++	++	-
2#	++	-	+	++	+	+
3#	+	+	+	++	++	+
4#	+	+	-	++	++	++
5#	+	+	-	++	+	+
6#	+	-	+	++	++	+
7#	++	++	-	++	+	++
8#	++	+	+	++	+	+
9#	++	++	+	+	-	-
10#	-	-	-	++	-	++
11#	++	++	++	++	+	+
12#	++	+	+	++	+	+
13#	++	++	+	++	+	++
14#	++	+	+	+	-	+
15#	++	+	-	++	++	+
16#	+	+	-	++	+	+
17#	++	+	+	++	+	++
18#	++	+	+	++	+	+
19#	++	-	+	++	++	+
20#	++	+	+	++	+	+
21#	++	++	+	++	++	+
22#	++	+	++	+	-	-
23#	++	+	++	+	-	+
24#	++	++	++	+	-	-
25#	+	-	+	++	+	++
26#	+	+	-	++	+	+
27#	+	+	+	++	++	++
28#	++	+	+	+	+	-
29#	++	+	++	++	++	++
30#	++	+	+	+	+	+

Table 7. Statistics of quality assessment results of 6 samples on 30 carriers

Yuan Zhuang, Shuhui Gao, Fei Xie, Wei Huang, Nengbin Cai. Identifying Bloodstain Species Using Hyperspectral Imaging[J]. Laser & Optoelectronics Progress, 2022, 59(16): 1630001

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