To evaluate the flicker visibility of active shutter type stereoscopic display more precisely and objectively, the temporal luminance response signal measurement based flicker visibility objective evaluation method is proposed. The temporal luminance measurement system realizes the precise record of display luminance output. Based on the critical flicker frequency (CFF) model of cathode ray tube (CRT) display, the perception experiments on stereoscopic display with active shutter glasses are implemented to establish the flicker visibility objective evaluation model of stereoscopic display with active shutter glasses. The correlation coefficient between the estimation result of the proposed model and the perception result is 0.97. The temporal luminance measurement system combined with the flicker visibility estimation model realizes the objective evaluation of the flicker in stereoscopic display with active shutter glasses, and it also helps to optimize the visual comfort level of stereoscopic displays.