Xiao Wang, Yuan Zhao, Feng Yang, Jianhua Yin. Fluorescence Microscopy System with High Speed Polarization Based on Electro Optical Modulator[J]. Acta Optica Sinica, 2017, 37(11): 1118001
- Acta Optica Sinica
- Vol. 37, Issue 11, 1118001 (2017)
Fig. 1. (a) Schematic of fluorescence microscopy system with high speed polarization; (b) control sequence of polarization image acquisition signal; (c) optical path of polarization controlling
Fig. 2. Polarization rotation module combining EOM with QWP
Fig. 3. (a) Polarization angle versus applied output voltage; (b) polarization states of distorted output linear polarization light from polarization control unit (green lines) at the back focal plane before (purple lines) and after (blue lines) compensation by Soleil-Babinet compensator
Fig. 4. (a) Schematics of lipid molecules at equatorial plane of giant vesicle; (b) fluorescence intensity under polarization angle of α; (c) fluorescence images recorded by CCD at various polarization angle α
Fig. 5. (a) Total fluorescence intensity image after summing up polarization fluorescence images; (b) fluorescence modulation responses and fitting modulation curves for four points A、B、C、D in Fig. 5(a); (c) average direction angle θ; (d) modulated amplitude R
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