We design a yellow light multi-wavelength tunable Raman laser by multi-stage stimulated Raman scattering in fiber and acousto-optic tunable filter, which can output a single wavelength. We get six wavelengths of yellow laser from 558 to 583 nm. Pulse energy of a single wavelength is about 5 μJ, the full-width at half-maximum of the laser is less than 5.4 nm, the pulse width is about 2.5 ns, and the divergence angle is less than 1\O. This work provides a new direction for new wavelength Raman tuning laser using tunable AOTF.