An all-fiber pulse laser based on master oscillator power amplification (MOPA) configuration is reported. Using the acousto-optic Q switch, the pulse laser seed with average power of about 500 mW is obtained. A pre-amplifier amplifies the seed power up to 10 W. The main amplifier is based on the Yb-doped double clad fiber pumped by a pigtailed laser diode with a center wavelength of 976 nm. In the experiment, pulse laser with repetition rate of 40 kHz at the center wavelength of 1064 nm is realized. The pulse width is 2.4 μs, the average power is greater than 100 W, and the corresponding pulse energy is 2.63 mJ. The M2 factor of output beam is 1.2.