The parameters of Tm3+ and Ho3+ ions in ZBLAN are given, and compared with Ω of Tm3+ and Ho3+ ions in oxide glasses. Under excitation of Tm3+ ion in the single Tm3+ doped ZBLAN by 357 nm (1D2) or 486 nm (1G4) wavelength beam, the luminescence intensities of the differeny emission wavelength from 1D2, 4G4 levels as a function of the doped concentretions have been obtained. When Tm3+ or Ho3+ ions of the single doped sample excited by <1 μm wavelength, the IR luminescence spectra are measured. The effect of ion concentration on the IR emission intensity and fluorescence lifetime of Tm3+ and Ho3+ ions were discussed. Tm3+ (3F4)→Ho3+ (5I7) energy transfer in(Tm3++Ho3+) co—doped ZBLAN glasses is very effective, its transfer efficiency is over 80% and dependent on the doped concentration.