Author Affiliations
1School of Mechanical and Electrical Engineering, Guangzhou University, Guangzhou, Guangdong 510006, China2Research Lab for Biomedical Optics and Molecular Imaging, Shenzhen Institutes of Advanced Technology,Chinese Academy of Sciences, Shenzhen, Guangdong 518055, China3Department of Ultrasound, the Third Affiliated Hospital, Sun Yat-Sen University,Guangzhou, Guangdong 510630, Chinashow less
Fig. 1. Schematic of high-resolution photoacoustic microscopy imaging system
Fig. 2. Spatial resolution test results of imaging system. (a) Fitted lateral profiles of blade edge; (b) photoacoustic MAP image of blade edge; (c) Gaussian-fitted axial profile of single carbon fiber; (d) MAP image of carbon fibers
Fig. 3. Imaging depth test results of system. (a) Photo of phantom; (b) photoacoustic imaging result
Fig. 4. High-resolution photoacoustic microscopic imaging results of micro-vessels in ear of healthy mouse. (a) Photo of mouse; (b) depth-encoded photoacoustic imaging result of ear vessels; (c) enlarged circle area in Fig. 4(b); (d) Gaussian-fitted profile of photoacoustic signal intensity along white line in enlarged area
Fig. 5. Steps and results of vascular signal extraction. (a) Flowchart of vascular signal extraction algorithm; (b) original image of mouse ear vessels (rectangle area in Fig. 4); (c) feature MAP of mouse ear vessels; (d) enhanced image of mouse ear vessels; (e) extracted vessels of mouse ear
Fig. 6. Effect comparison of tumor vessel extraction. (a) Photoacoustic image of tumor vessels; (b) enlarged image of red dashed rectangle area in Fig. 6(a); (c) processing result of 2D Hessian matrix algorithm; (d) processing result of 3D Hessian matrix algorithm
Fig. 7. Continuous unmarked monitoring results of tumor angiogenesis in ear of mouse. (a)-(c) Depth-encoded photoacoustic imaging results of tumor vessels on day 0, day 2, day 4; (d)-(f) MAPs of extracted vessels corresponding to Figs. 7(a)-(c); (g) quantitative results of vessel density on different days; (h) quantitative results of vessel tortuosity on different days; (i) quantitative results of vessel diameter Dv distribution on different days
Fig. 8. Continuous unmarked monitoring changes of mouse tumor vessels response to anti-angiogenic therapy. (a)-(d) Depth encoded photoacoustic imaging results of tumor vessels on day 0, day 4, day 5, day 6; (e)-(h) MAPs of extracted vessels corresponding to Figs. 8(a)-(d); (i) quantitative results of vessel density on different days; (j) quantitative results of vessel tortuosity on different days; (k) quantitative results of vessel diameter Dv distribution on different days