Based on naked-eye three-dimensional display technology with LED screen, this paper proposes a method for improving the reconstruction spatial resolution of integral stereo imaging system by using time division multiplexing technology, which solves the problem of low resolution caused by the divergence of the pixel point source of the LED screen and the excessive spacing between the adjacent pixels. By studying the illumination principle of LED display devices, we analyze the factors affecting the spatial resolution of the reconstructed image and the influence factor of reconstruction resolution of imaging system is obtained. Therefore, when the LED display array is unchanged, the time division multiplexing technique can effectively extract the pixel information around a single pixel point and finally improves the spatial resolution of the reconstructed image. The theoretical analysis and experimental verification of this method show that the proposed method effectively improves the resolution of the reconstructed image of LED display and meets the requirements of improving the resolution of integral imaging reconstruction.