Multi-Focus Image Fusion Method for Microscopic Algal Images

Renqing Jia; Gaofang Yin; Nanjing Zhao; Min Xu; Xiang Hu; Peng Huang; Tianhong Liang; Yu Zhu; Xiaowei Chen; Tingting Gan; Xiaoling Zhang

doi:10.3788/AOS222153

Journals >Acta Optica Sinica >Volume 43 >Issue 12 >Page 1210001 > Article

Acta Optica Sinica
Vol. 43, Issue 12, 1210001 (2023)

Multi-Focus Image Fusion Method for Microscopic Algal Images

Renqing Jia^1、2, Gaofang Yin^2、*, Nanjing Zhao^1、2、**, Min Xu², Xiang Hu³, Peng Huang³, Tianhong Liang², Yu Zhu⁴, Xiaowei Chen², Tingting Gan², and Xiaoling Zhang⁵

Author Affiliations

¹School of Environment Science and Optoelectronic Technology, University of Science and Technology of China, Hefei 230026, Anhui, China

²Key Laboratory of Environment Optics and Technology, Chinese Academy of Sciences, Anhui Institute of Optics and Fine Mechanics, Hefei Institutes of Physical Science, Chinese Academy of Sciences, Hefei 230031, Anhui, China

³Hefei University, Hefei 230601, Anhui, China

⁴Anhui Ecological Environment Monitoring Center, Hefei 230061, Anhui, China

⁵Anhui University, Hefei 230601, Anhui, China

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DOI: 10.3788/AOS222153 Cite this Article Set citation alerts

Renqing Jia, Gaofang Yin, Nanjing Zhao, Min Xu, Xiang Hu, Peng Huang, Tianhong Liang, Yu Zhu, Xiaowei Chen, Tingting Gan, Xiaoling Zhang. Multi-Focus Image Fusion Method for Microscopic Algal Images[J]. Acta Optica Sinica, 2023, 43(12): 1210001 Copy Citation Text

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Fig. 1. Flow of proposed microscopic multi-focus image fusion method

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Fig. 2. Anabaena sp. multi-focus microscopic images obtained by moving the platform to 7 μm

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Fig. 3. Scenedesmus sp. multi-focus microscopic images obtained by moving the platform to 7 μm

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Fig. 4. Pediastrum sp. multi-focus microscopic images obtained by moving the platform to 15 μm

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Fig. 5. Visual comparison of fusion results for WT, LP, PCNN, and proposed methods

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Fig. 6. Comparison of residual errors of WT, LP, PCNN, and proposed methods

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Sample	Indicator	WT	LP	PCNN	Proposed
Anabaena sp.	Entropy	5.6142	5.4930	5.7676	5.9804
	Average gradient	0.0028	0.0030	0.0038	0.0055
	Spatial frequency	4.5093	4.8050	6.1187	8.9654
	Standard deviation	5.7135	5.0338	7.4390	6.0689
	$Q^{A B / F}$	0.2683	0.2275	0.2261	0.3529
	Time /s	5.55	1.83	52.70	2.23
Scenedesmus sp.	Entropy	6.4140	6.5952	6.4580	6.5273
	Average gradient	0.0013	0.0015	0.0015	0.0023
	Spatial frequency	3.9649	4.4937	4.7897	7.0558
	Standard deviation	7.7412	7.6470	8.5040	7.7291
	$Q^{A B / F}$	0.3290	0.3141	0.2750	0.3778
	Time /s	5.50	1.82	47.50	2.33
Pediastrum sp.	Entropy	4.0154	3.9114	3.9404	4.0134
	Average gradient	0.0004	0.0004	0.0004	0.0005
	Spatial frequency	1.3795	1.3187	1.4559	1.5445
	Standard deviation	12.9384	12.6871	15.1042	13.6266
	$Q^{A B / F}$	0.2348	0.2003	0.2030	0.2940
	Time /s	4.70	1.70	45.10	2.10

Table 1. Comparison of objective indicators for WT, LP, PCNN, and proposed methods

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