A new approach to measure the temperature of large-scaled cold atomic cloud is reported. In this approach, a pair of anti-Helmholtz coils is used to generate a quadrupole magnetic field, the spatial distributions of cold atoms in different moments are measured by applying a vertical probe beam which can detect the density of cold atomic cloud point by point, and then the temperature of cold atoms is fitted from the experimental results. The temperature of cold atoms in integrating sphere is demonstrated to be 73±12 μK by using this approach. The result is also compared with time-of-flight (TOF) method.