The contents of inorganic elements including K, Ca, Na, Mg, P, S, Fe, Cu, Mn, Zn, Mo, and Co in the soil-grass-animal mineral system from Qinghai Tibetan Plateau were determined by ICP-AES using high pressure system-sealed microwave digestion. The sample of soil was digested with HNO3-HF-H2O2 acids system, but others including pasture, animal fur, liver, and serum were digested with HNO3-H2O2 acids system. The operation would be simplified and the blank value would be decreased with the above acids systems. The results were proved to be reliable with the relative standard deviation being 0.271%-2.633% for Ca, 2.971%-4.854% for Co, 0.372%-2.874% for Cu, 0.600%-3.683% for Fe, 0.347%-2.829% for K, 0.626%-2.593% for Mg, 0.705%-4.828% for Mn, 2.946%-4.622% for Mo, 0.689%-3.621% for Na, 0.422%-3.890% for P, and 0.143%-4.622% for S, 0.166%-2.399% for Zn, and all of them were less than 5% for all the elements, and the recovery being 97.1%-101.4% for Ca, 93.5%-112.5% for Co, 95.2%-104.0% for Cu, 96.9%-104.2% for Fe, 96.1%-105.6% for K, 96.2%-102.8% for Mg, 91.5%-108.9% for Mn, 95.0%-113.5% for Mo, 95.2%-101.8% for Na, 94.7%-100.7% for P, 98.3%-108.4% for S, and 97.5%-102.0% for Zn by adding standard recovery experiment. The results of determination were proved that the method of sealed microwave digestion ICP-AES was sensitive, precise, easy to operate and rapid for the determination of inorganic elements in the soil-grass-animal mineral system, and could satisfy the sample examination request. The methods and results were meaningful to research on the soil-pasture-animal mineral system including the contents of mineral elements, the circulation of mineral elements, the interaction, and the application of mineral additive.