Thiazone, a kind of new enhancer, is introduced to investigate the effect of optical clearing on skin tissue. The samples including skin with or without stratum corneum (SC), are treated with pure polyethyleneglycol 400 and mixed solution (thiazone in combination with polyethyleneglycol 400) respectively. The transmittance of light with wavelength 632.8 nm is measured dynamically by using an integrating sphere system, and then photos of the samples are taken by CCD. Experimental results show that mixed solutions have optical clearing effect on both kinds of skin, The change in attenuation coefficient of skins without SC is 19 times of that with SC. Single PEG400 can only do some effect on the sample without SC, and the change in attenuation coefficient of samples is just 2/3 of that treated by mixed solution. It is illustrated that thiazone can increase the effect of optical clearing on skin, because it is an efficient enhancer and has synergistic effects with PEG400.