The Detection of fruit contaminate with pesticide residue is a public health concern. The pesticide was diluted to 1:20, 1:100 and 1:1000 solution with distilled water. A 3×3 matrix of dilutions was applied to 10 cleaned sample with 120 μL, 200 μL and 400 μL. After 168h, hyperspectral images in the wavelength range from 625 nm to 725 nm are taken. The characteristic wavelength images are achieved by principal component analysis (PCA). Combining the 3rd principal component (PC) image with proper image processing methods detects the pesticide residue on navel orange. This research shows that the technology of hyperspectral imaging can be used to effectively detect pesticide residue on fruit surface.